BRG1 Recombinant Rabbit Monoclonal Antibody [SN20-03]
Recombinant Rabbit monoclonal Antibody
Synthetic peptide within human BRG1 aa 240-280.
Human, Mouse, Rat (Predicted: Pig)
WB, IF-Cell, IF-Tissue, IHC-P, IHC-Fr, FC
Predicted band size: 185 kDa
HeLa, HeLa cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, HepG2, NIH/3T3, human tonsil tissue, human kidney tissue, human breast carcinoma tissue, mouse testis tissue, mouse colon tissue, mouse kidney tissue, mouse epididymis tissue, rat colon tissue, rat kidney tissue, rat brain tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.
unconjugated
SN20-03
Liquid
1ug/ul
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
IgG
Protein A affinity purified.
WB
1:20,000-1:50,000
IF-Cell
1:200-1:1,000
IHC-P
1:1,000-1:5,000
IF-Tissue
1:200-1:500
IHC-Fr
1:1,000
FC
1:1,000
| Human | 查看 1 篇文献如下 |
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
1. Madsen MS et al. Peroxisome proliferator-activated receptor and C/EBPa synergistically activate key metabolic adipocyte genes by assisted loading. Mol Cell Biol 34:939-54 (2014).
2. Ramos P et al. Small cell carcinoma of the ovary, hypercalcemic type, displays frequent inactivating germline and somatic mutations in SMARCA4. Nat Genet 46:427-9 (2014).
Belongs to the SNF2/RAD54 helicase family.
Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
Nucleus.
ATP dependent helicase SMARCA4 antibody
ATP-dependent helicase SMARCA4 antibody
BAF 190 antibody
BAF190 antibody
BAF190A antibody
Brahma protein like 1 antibody
BRG1 antibody
BRG1 associated factor 190A antibody
BRG1 protein antibody
BRG1-associated factor 190A antibody
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Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: Not required
Application: IHC-Fr
Species: Mouse
Site: E14.5 embryo
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Application: IHC-Fr
Species: Mouse
Site: Testis
Sample: Frozen section
Antibody concentration: 1:1,000
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
☑ Relative expression (RE)
Western blot analysis of BRG1 on different lysates with Rabbit anti-BRG1 antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: A549 cell lysate (negative)
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
FBXO42 facilitates Notch signaling activation and global chromatin relaxation by promoting K63-linked polyubiquitination of RBPJ
Author: Jiang, H., Bian, W., Sui, Y., Li, H., Zhao, H., Wang, W., & Li, X.
PMID: 36129980
期刊: Science Advances
应用: WB
反应种属: Human
发表时间: 2022 Sept
Citation
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