HRP Conjugated Goat anti-Rabbit IgG polyclonal Antibody
Goat Polyclonal Antibody
Rabbit IgG (H+L).
To minimize cross-reactivity, the HRP conjugated goat anti-rabbit IgG (H+L) antibodies have been highly cross-adsorbed against human IgG and Mouse IgG.
Rabbit
WB, ELISA, IHC-P
HRP
Liquid
2ug/ul
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.5%BSA, 50% Glycerol.
IgG
Immunogen affinity purified.
WB
1:50,000~1:100,000
ELISA
1:5,000~1:10,000
IHC-P
1:100-1:200
| Human | 查看 157 篇文献如下 |
| Mouse | 查看 137 篇文献如下 |
| Rat | 查看 46 篇文献如下 |
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
暂无
Western blot analysis of rabbit anti-GAPDH antibody (ET1601-4,1/2000) and rabbit anti-GST3 antibody (ER1802-63, 1/1000) with K562 Cell and A549 Cell lysates (All lanes proteins at 10 µg per lane). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat anti-rabbit IgG -HRP) (HA1001) was used for 1 hour at room temperature at 1:40,000 (Lane A), 1:80,000 (Lane B), 1:160,000 (Lane C) and 1:320,000 (Lane D).
Exposure time: 60 seconds
The Cross-reactions of the secondary antibody (HA1001) with Mouse IgG and Human IgG.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Goat anti-rabbit IgG -HRP (HA1001) was used for 1 hour at room temperature at 1:40,000 (Lane A) and 1:180,000 (Lane B).
Exposure time: 2 minutes
Identification of a novel FOXO3‑associated prognostic model in hepatocellular carcinoma
Author: Songmei Guan, Qiang Lin, Peiwu Huang, Kangqiang Lin, Shigang Duan
PMID: 40114746
期刊: Oncology Letters
应用: WB
反应种属: Human
发表时间: 2025 Mar
Copyright © 广州杰特伟生物科技有限公司 All Rights Reserved. 备案号：粤ICP备19077843号
