HA tag Rabbit Polyclonal Antibody
Rabbit Polyclonal Antibody
Synthetic peptide immune sequence is YPYDVPDYAC.
Species independent
WB, IP
C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein.
unconjugated
Liquid
1ug/ul
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
IgG
Immunogen affinity purified.
WB
1:5,000-1:10,000
IP
2-5 µg/ml.
| WB | 查看 30 篇文献如下 |
| IP | 查看 4 篇文献如下 |
| Co-IP | 查看 3 篇文献如下 |
| CO-IP | 查看 2 篇文献如下 |
| IHC | 查看 2 篇文献如下 |
| IF | 查看 1 篇文献如下 |
| COIP | 查看 1 篇文献如下 |
| CoIP | 查看 1 篇文献如下 |
| FC | 查看 1 篇文献如下 |
| co-IP | 查看 1 篇文献如下 |
| Human | 查看 19 篇文献如下 |
| Mouse | 查看 4 篇文献如下 |
| Zebrafish | 查看 2 篇文献如下 |
| Human hepatocellular carcinoma cell | 查看 1 篇文献如下 |
| HEK293T | 查看 1 篇文献如下 |
| HEK293 cells | 查看 1 篇文献如下 |
| Escherichia coli | 查看 1 篇文献如下 |
| None | 查看 1 篇文献如下 |
| Species independent | 查看 1 篇文献如下 |
| Persimmon fruit | 查看 1 篇文献如下 |
| Nicotiana benthamiana | 查看 1 篇文献如下 |
| Kiwifruit | 查看 1 篇文献如下 |
| rice | 查看 1 篇文献如下 |
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.
1. Cao, H., Yang, C., Quan, S., Hu, T., Zhang, L., Zhang, Y., Liu, Q. (2018). Novel T3SS effector EseK in Edwardsiella piscicida is chaperoned by EscH and EscS to express virulence. Cell Microbiol, 20(1). PMID: 29024267
2. Zhou, Y., Xiong, L., Zhang, Y., Yu, R., Jiang, X., & Xu, G. (2016). Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells. J Proteomics, 148, 94-104. PMID: 27432471
3. Wang, K., Jin, X., Chen, Y., Song, Z., Jiang, X., Hu, F., Topping, D. L. (2016). Polyphenol-Rich Propolis Extracts Strengthen Intestinal Barrier Function by Activating AMPK and ERK Signaling. Nutrients, 8(5).PMID: 27164138
4. Fu, G., Xu, Q., Qiu, Y., Jin, X., Xu, T., Dong, S., Lu, L. (2017). Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. J Exp Med, 214(5), 1453-1469. PMID: 28400474
5. You, Q., Wu, Y., Yao, N., Shen, G., Zhang, Y., Xu, L., Ju, C. (2015). Interaction of AIM with insulin-like growth factor-binding protein-4. Int J Mol Med, 36(3), 833-838.
HA Tag
Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal HA-tagged recombinant protein
Lane 2: N-terminal HA-tagged recombinant protein
HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with 0906-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 60 mins at room temperature.
Lane 1: HA Tag fusion protein lysate (input).
Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.
Lane 3: 0906-1 IP in HA Tag fusion protein lysate.
Lane 4: Rabbit IgG instead of 0906-1 in HA Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
期刊: Cell Reports
应用: IP,WB
反应种属: Human
发表时间: 2025 Mar
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